Removal of perineuronal nets leads to altered neuronal excitability and synaptic transmission in the visual cortex with distinct time courses

Abstract

Parvalbumin-expressing (PV) interneurons fast inhibit excitatory neurons in various brain areas. Perineuronal nets (PNNs), accumulating around PV neurons, have been shown to play critical roles in neuronal function and plasticity. The cellular mechanisms underlying their functions are still in debate, for example, do PNNs contribute significantly to the excitability of inhibitory neurons especially those containing PV? On the other hand, whether PNNs have significant contributions to synaptic transmission of PV neurons is much less unknown. In this study, we designed experiments to address these questions and found that removing PNNs in vivo using chondroitinase ABC (ChABC) led to distinct changes in neuronal excitability and synaptic transmission, depending on the duration of ChABC treatment. The results showed 7 days after ChABC treatment reduced both intrinsic excitability of PV neurons and synaptic transmission to both PV neurons and excitatory neurons in the primary visual cortex. However, 1 day after ChABC treatment digested PNNs effectively but had no effects on intrinsic excitability and synaptic transmission. These results suggest the contribution of PNNs to neuronal excitability and synaptic transmission depends on different time courses of ChABC digestion.