Abstract
We investigated the effect of removal of hydroxyl radical on nitric oxide (NO)-induced [ 3H]GABA release from cerebral cortical neurons. NO generators, S-nitroso- N-acetylpenicillamine and sodium nitroprusside, significantly increased [ 3H]GABA release, which was completely abolished by hemoglobin. These results indicate that the release of [ 3H]GABA evoked by these NO generators is mediated by NO formation. N,N′-Dimethylthiourea (DMTU), a hydroxyl radical scavenger, dose-dependently stimulated the increase of NO-induced [ 3H]GABA release. Similarly, other hydroxyl radical scavengers such as uric acid and mannitol, significantly enhanced the NO-induced [ 3H]GABA release. On the other hand, each hydroxyl radical scavenger alone showed no effect on [ 3H]GABA release in the absence of NO generators. These results indicate that the removal of hydroxyl radical enhances NO-evoked [ 3H]GABA release from cerebral cortical neurons.
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