Abstract

A two-phase solvent extraction system for the removal of glucosinolates from Midas (high-glucosinolate) rapeseed and mustard seed was studied. The polar phase consisted of different levels of ammonia in methanol containing 5% water, and hexane was used as the non-polar second phase. The effect of a quiescent contact period, the concentration of ammonia, and the ratio of the volume of the methanol/ammonia phase to the weight of seed (R) on the glucosinolate content of the meal was studied. The optimum conditions for glucosinolate removal at room temperature were a 30-min quiescent period, 10% ammonia concentration, and R = 6.7. Over 98% of all glucosinolate was removed after a second extraction of the resultant meal with the above extraction system under optimum conditions.

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