Removal of cell-associated myxovirus by antibody.

Abstract

Discussion and SummaryThe fact that the two strains IS and BA in AL showed such a large difference in sensitivity to the same NDV antibody makes it seem likely that the disappearance of cell-associated viral hemagglutinins is due to a reaction at the cell membrane, not inside the cell. Since the HAI titer was approximately the same for IS as for BA, entry of antibody into the cell should cause equal inhibition. In the antibody-sensitive cell-virus systems, virus budding through the membrane probably is removed as fast as it is formed and it follows that maturation is exclusively at a site accessible to antibody. With virus strains where large amounts of cell associated hemagglutinins are formed, even in the presence of extracellular antibody, intracellular HA production seems likely. Evidence for association of influenza virus with internal membranes of the cell has been presented by Holland and Kiehn (8). Also it is of interest that ascites lymphoma cells infected with influenza are more resistant to ant...