Removal of carboxymethyl cellulase activity from the culture filtrate of Termitomyces clypeatus producing xylanase

Abstract

Termitomyces clypeatus produced 450 IU xylanase ml−1 in a medium containing starch-free wheat bran powder as the carbon source. Carboxymethyl cellulase (CMCase) activity in the culture filtrate was removed by keeping the filtrate at pH 10 for 60 min followed by a change to pH 6. Treatment of Kraft pulp (bamboo) with the filtrate at pH 7 decreased the kappa number from 10.5 to 5 with release of reducing groups equivalent to 0.15 mg glucose g−1 pulp.