Abstract
Ascorbic acid, which depresses the colour and interferes in reactions in colorimetric and fluorometric assay procedures for hydrogen peroxide involving horseradish peroxidase, has been removed from solutions by treatment with an iodine reagent. This reagent strongly interferes with procedures involving scopoletin, 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) and the reaction between 3-dimethylaminobenzoic acid and 3-methyl-2-benzothiazolinone hydrazone. The iodine reagent slightly depressed the fluorescence obtained using hydroxyphenylacetic acid but this was the most suitable electron donor for amounts of hydrogen peroxide between 6 and 60 nmol. Hydroxyphenylacetic acid spontaneously reduces residual iodine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
