Abstract
We demonstrated the feasibility of Raman spectroscopy for remote temperature monitoring within the aqueous humor of the rabbit eye in vivo. Using a confocal Raman spectroscopy system, Raman spectra from 2580 to 3800 cm(-1) were recorded in HPLC-grade water and in the aqueous humor of the rabbit eye under in vivo and ex vivo conditions within a temperature range of 14-34 degrees C. The ratio between the integrated Raman intensities of two temperature dependent OH-vibrational regions (OH2/OH1) in the spectra of water showed high linear dependence on temperature both in pure water [0.0049(+/-1.2%)T+0.4522(+/-0.5%), R2=0.99, n=50, p<0.05], as well as in the rabbit aqueous humor [0.0036(+/-2.8%)T+0.4966(+/-0.6%), R2=0.98, n=162, p<0.05] with a high degree of reproducibility and sensitivity ( approximately 0.2-0.7 degrees C). Raman spectroscopy can be used for high resolution and remote monitoring of temperature in the aqueous humor under in vivo conditions.
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