Remineralization of initial enamel like lesions with chicken Egg Shell powder solution versus Amorphous Calcium Phosphate

Abstract

Aim: To compare the efficacy of chicken egg shell solution powder (CESP) and amorphouscalcium phosphate (ACP) on the remineralization of enamel subsurface lesions.Materials and Methods: Twenty bovine central incisor teeth were used in this study. Themicrohardness and the minerals content of all specimens were initially assessed using Vickershardness tester and EDXA respectively (positive control group).The specimens were artificiallydemineralized and then reassessed directly after demineralization (negative control group). Alldemineralized specimens were randomly classified into 2 equal groups,group I :treated with Chickeneggshell powder solution (10 specimens), while, groupII: treated with ACP (10 specimens). Theremineralizing agent were applied twice daily for 15 min each for seven successive days. Finally allspecimens were reassessed for minerals content (Ca and PO4 weight %) and surface microhardness.The data were statistically analyzed using repeated measures analysis of variance (ANOVA ), thesignificance level was set at P ≤ 0.05.Results: Microhardness was significantly decreased in all specimens after demineralizationand then it was significantly increased after exposure to therapeutic solutions. There was asignificant increase (P<0.001) in both Calcium and Phosphorus levels after remineralization usingboth agents, as detected by EDAX. However, there was no significant difference between the tworemineralized groups.Conclusions: Within the limitations of this in vitro study, it can be concluded that bothremineralizing agents were similarly able to increase the microhardness and tooth remineralization.However, being natural products, CESP can be considered as an optimal alternative to thecommercial ones.