Remineralization Effect of Two Types of Varnishes on Induced Enamel Caries

Abstract

Purpose: to assess the remineralization capacity of MI varnish and Bifluorid 10 varnish on induced white spot lesions in deciduous enamel, using Scanning Electron Microscope- Energy Dispersive X-ray. Materials and methods: Sixty human caries-free deciduous teeth were collected. The teeth were stored in 0.1% thymol solution. A 4*4*2 window was created on the buccal surface of each tooth using acid-resistant nail varnish. Samples were then divided into 3 groups according to the remineralizing agent used, each group containing 20 teeth. Group I was remineralized with MI varnish, group II was remineralized using Bifluorid 10 varnish, while group III was a control group that didn’t receive any remineralization. Then, baseline assessment of Calcium/Phosphorous (% weight) was done using SEM-EDX. Teeth were then immersed in a demineralizing solution at 37°C for 72 hours, until white spot lesions in enamel were visible. SEM-EDX assessment was carried out for the demineralized samples. Remineralization using MI varnish and Bifluorid 10 varnish was done in groups I and II respectively. SEM-EDX assessment was carried out to evaluate the remineralization capacity of each varnish. Results: SEM-EDX examination showed that MI varnish had the highest remineralization capacity. The examination showed statistically significant difference between group I and II. They are both superior to the control group. Conclusion: Both MI varnish and Bifluorid 10 varnish had acceptable remineralization capacity. MI varnsih is preferred.