Remembering the order of serially presented objects: A matter of time?

Abstract

Remembering the sequence, in which stimuli are encountered or events have occurred, is a key process in episodic memory and can also facilitate recognition memory. Rodents, when presented with a sequence of objects, will explore the object encountered first; yet, whether this behaviour is because the rodents spontaneously encode the order of stimuli presentation or because of relative familiarity or temporal decay is unknown. Here, we tested sequence memory in rats using a series of spontaneous preference tasks. Experiment 1 demonstrated that when rats are presented with a sequence of four objects, with an inter-sample interval of 5 min or 1 h, they preferentially explored the object presented earlier in the list irrespective of the inter-sample interval. We then demonstrated that such memory for order was not affected by increasing or decreasing the inter-sample interval between the middle objects (Experiment 2). Finally, we showed that memory for order is not a function of absolute object familiarity, as animals showed clear discrimination between the objects presented in the sample phases and a novel object, independent of the sample objects’ position in the sequence (Experiment 3). These results show that animals are able to encode the order of objects presented in a sequence, and as such temporal order memory is not achieved using the process of relative or absolute familiarity or temporal decay.