Abstract
Batch reactors and microcosms were used to evaluate groundwater bioremediation potential of tetrachloroethene (PCE) in the presence of additional pollutants present at a site located in the Apulia Region (SE Italy). Reductive dechlorination of PCE was studied under anaerobic conditions by comparing the effectiveness of three inocula: (a) soil sampled at the contaminated site, (b) anaerobic sludge from a municipal wastewater plant, and (c) an enriched dehalogenating culture containing Dehalococcoides species. In order to enhance dehalogenation, reactors inoculated with sludge were also amended with selected electron donors. Aerobic reactors were also established to study oxidative degradation of vinyl chloride (VC), that may accumulate after incomplete dechlorination of PCE. Results showed that consortia derived from anaerobic sludge and amended with electron donors quantitatively and incompletely degraded PCE to cis-dichloroethylene, whereas in reactors augmented with a dehalogenating culture complete dechlorination of PCE occurred even in the presence of additional toxic contaminants. The presence of Dehalococcoides spp. in the dehalogenating culture and its absence in reactors inoculated with anaerobic sludge was confirmed using FISH community analyses. In all cases, prolonged incubation periods were necessary for dechlorination. On the other hand, oxidative degradation of VC in aerobic reactors occurred after short lag times.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
