Remediation of Heavy Metal Ions of Cadmium, Lead And Mercury From Its Salts Solution, By Precipitating Out Using Biological Fluid Indian Buffalo’s (Bubalusarnee) Milk Subsequent Analysis of Filtrate By Icp-Aes And X Ray Fluorescence of White Flocculent Precipitate Containing Milk Protein and of Filtrate Solution.

Abstract

Boiled buffalos milk has been used to precipitate out metal ions of Cd,Pb and Hg from its salt solution of nitrates. The white flocculent containing metal ion and milk protein has been filtrated out the white flocculent after several wash was dried tested by Xray Fluroescence for the presence of these metals in the white flocculent. Xray Fluroescence results confirm the presence of these metal in the white flocculent .ICP-AES analysis for the filtrate solution have also been carried out for quantitative measurement of metal ion in the filtrate solutions ICP-AES results show for cadmium ion from conc of 450ppm in control solutions it becomes 95ppm,in case of Pb ion Control conc of 960ppm it becomes 15ppm and for Mercury control conc of 21660ppm it becomes 500ppm after addition of 20ml aliquot of boiled buffalo milk in 100ml of metal ions solution. The dilution effect taken in to account reduction in the metal ions conc from control solutions actually goes to the white Flocculent Milk protein by replacing Ca from the original Milk protein by toxic heavy metal ions a theory has been proposed based on the std reduction potential chart of metal ions. Thus biological fluid buffalos milk which is available in plenty in the world can be used for replacing synthetic reagents thus checking the environmental pollution thus can emerge as one of the best Green chemistry reagents and it may help in the metal processing industries of these toxic metals to ease pollution. I. INTRODUCTION. Last 300 years with rapid growth of science and technology human race thought they have able to manipulate many things in the nature and make life better, however soon it has been realized the anthropogenic activities(1) leads different kind of environmental pollution it may be air pollution, water pollution , earth pollution or space pollution. Now we cannot go back to primitive stage nor we can advance in the same direction , so a middle path approach which can keep essence of modern science at the same time use natural substances which is easily available around us for utilization to protect the nature is being envisaged allover the world like for example currently the emergence of green chemistry concept (2) where the processes are being made more natural instead of final product recycling has been given top most thought. One such approach for remediation of toxic heavy metal ions Cd, Pb and Hg from its salt solution of nitrites using easily available Indian buffalos milk has been tried. In the last article of the author in the same journal cows urine have been utilized to precipitate out these toxic heavy metals has been reported(3). The use of biological fluids such as cows urine or buffalos milk for remediation of toxic heavy metals has never been reported in internet search or else where , thus it opens new vistas for environmental pollution control. The reason of trying buffalos milk is not throwing stone in the dark which may hit the target but with proper scientific basis and understanding. Milk contains protein and Buffalos milk contain maximum protein as compared to cows , Goat and Camel milk . The protein has got natural affinities for these toxic heavy metals(4) especially because of sulfydryl groups - SH like in protein cysteine which is found in the milk(5) II. Experimental. Indian Buffalo (Bubalus ar nne) milk have been procured from local Buffalo shed. Milk was boiled to make it condense till the color changes to slightly reddish hue. The nitrate salts of Cadmium . Lead and Mercury was procured from local sd fine chemicals,Mumbai, India. The solutions of these heavy metal salts were made in nano pure water. The conc.of heavy metal ions in the solutions were of range 460ppm to 21000ppm. for Cd,Pb and Hg. In 100ml of the aliquot of these solutions 20ml of boiled buffalo milk were added. The addition of milk leads to precipitation of white flocculent protein material just like milk splitting phenomena but in this case white flocculent sinks at the bottom within 5-10 minutes. of addition of milk. The settling of white flocculent protein material was separated from the rest of the solution by filtrating out. The white flocculent