Abstract
Yeast was used to prepare permeable reactive barrier (PRB) with immobilized microbial technology, and the electrokinetics coupled with the immobilized yeast PRB (IMEK-PRB) was established to remediate Cd-contaminated soil. The effect of the different PRBs prepared by immobilized microbial technology on Cd removal was explored. The voltage gradient had influence on the removal of Cd, and the removal reached as high as 53.70 % at a voltage gradient of 2.5 V/cm. The lowest removal about 34.12 % was obtained with yeast pellets prepared by the embedding method used as PRB. The yeast in PRB was partially broken and adhered, and the intensity of the absorption peak of the group analyzed with infrared spectra and the crystal diffraction peak from X-ray diffraction changed, leading to a decrease in its activity. The average removal of Cd increased by >10 % when fly ash-based yeast pellets prepared with the adsorption-embedding method, and fly ash-adsorbed yeast prepared by the adsorption method were used as PRB. IMEK-PRB remediation would greatly reduce the toxicity of Cd-contaminated soil, weaken harmful effects on the soil environment and reduce environmental risks. The fly ash-based yeast pellets used in IMEK-PRB have great application prospects for the remediation of Cd-contaminated soil.
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