Relief of Oxidative Stress Using Curcumin and Glutathione Functionalized ZnO Nanoparticles in HEK-293 Cell Line.

Abstract

To elucidate the effect of zinc oxide nanoparticles (ZnO-NPs) with different surface modifications in relieving the oxidative stress in cultured human embryonic kidney cells (HEK-293) following investigation was performed. Oxidative stress was artificially induced by hydrogen peroxide in HEK-293 cell culture and its management was studied. Alkyl amines modified ZnO-NPs with curcumin and reduced glutathione (GSH) functionalization was used in managing oxidative stress and had shown promising results. ZnO-NPs used in this study were synthesized via non-aqueous sol-gel method and FESEM characterisation showed them of spherical shape of about 20-50 nm size with amine, curcumin and GSH functionalization. UV-visible and FTIR spectroscopic characterizations confirmed functionalization of ZnO-NPs. Decrease in oxidative stress was found with the dose-dependent culture of HEK-293 cells with these functionalized ZnO-NPs. Cell viability and morphology, as observed using AFM and inverted microscope, was retained with the prescribed dosages of the functionalized nanoparticles while at higher dosages they caused cytotoxicity and death. Diethylamine (DEA) modified ZnO-NPs and their functionalization with GSH and curcumin were found more effective in managing oxidative stress in cells. Present study could help in designing economical and bio-compatible functionalized non-toxic nanoparticles designed for managing oxidative stress leading to possible therapeutical and medicinal uses.