Abstract
Because of the recent progress in lipid research, the availability of an excellent separation method for fatty acid methyl esters has become more and more important. A speical need exists for a good separation of cis and trans isomers, and of isomers with different positions of double-bonds. This goal was achieved by applying a 50 m glass-capillary column with an inner diameter of 0.33 mm, installed in a Carlo Erba Fractovap 2301 AC. The GC was equipped with FID, linear temperature programmer and automatic injection-system. The main characteristics of the system were a separation number of 47 and a theoretical plate number of 227052. The reproducibility of relative retention time and of weight %, expressed as maximum percent deviation of the mean values of 10 chromatograms was 0.26% and 1.53%, respectively. Preliminary results of the quantitative analysis of free cholesterol with a short OV-17 glasscapillary column, indicate a precision better than 0.8%.
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