Abstract
Monoclonal antibodies in triple-antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) and polyclonal antibodies in double-antibody sandwich ELISA (DAS-ELISA) were used for detection of three serotypes of barley yellow dwarf viruses (BYDVs) in field collected oat and wheat leaf samples. Agreement between the two ELISA systems was >99% for 900 and 1,050 samples collected in 1989 and 1990, respectively, indicating that the monoclonal antibodies were detecting highly conserved viral epitopes (...)
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