Abstract
In this study, we represented a new, simple, and highly sensitive method for determination of chlorhexidine di-gluconate (CHG) based on the fluorescence quenching of EDTA-Terbium (EDTA-Tb) probe in aqueous solution at λex 240 nm and λem 545 nm in presence of studied drug due to formation of ternary complex EDTA-Tb-CHG. A linear relationship between the concentration of the studied drug and the quenching effect on the EDTA-Tb complex was found over concentration range 2–35 ng/mL with a limit of detection (LOD) of 0.52 ng/mL and limit of quantification (LOQ) of 1.60 ng/mL with mean % recovery ± RSD of 100.61 ± 2.47. The approach was successfully applied for the determination of CHG in three different pharmaceutical preparations; mouth wash, antiseptic solution, and skin cleansing solution with mean % recovery ± RSD of 99.54 ± 2.75, 98.62 ± 1.65, and 99.66 ± 3.00, respectively. The method was validated according to ICH guidelines for the determination of the drug in its pure form and dosage form. The developed data was found to be in good agreement with the official method.
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