Abstract
The practical possibility of extracting static and dynamic parameters from fluorescence anisotropy decays of rod-like probes in membrane vesicles and other macroscopically isotropic samples with a locally ordered structure, such as cubic liquid crystals or unoriented polymer liquid crystals is discussed. Particular attention is paid to the obtainment of the fourth-rank order parameter 〈P4〉. For monodomain samples, global target analysis of samples at different temperatures has proved helpful. To test if this is true here as well, simulated single-photon-counting intensities at various temperatures have been prepared using a mean-field model and then analysed both individually and globally by means of target analysis. The results show that there is little hope of obtaining 〈P4〉 from macroscopically unoriented systems, except for some limiting cases. On the other hand, the recovery of 〈P2〉 and of the tumbling diffusion coefficient, D⊥, seems to be quite insensitive to the selected model for the anisotropic potential.
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