Abstract
Background. Analysis of γ-H2AX foci is a promising approach to evaluate exercise-induced DNA damage. However, baseline levels and day-to-day variability of γ-H2AX foci have not been investigated in healthy subjects at rest. Methods. Blood was taken from eight moderately trained healthy males (29 ± 3 yrs, 1.84 ± 0.03 m, and 85 ± 6 kg) at two separate days (M1/M2) after 24-hour exercise cessation. Number of γ-H2AX foci per 100 lymphocytes (N), number of foci per affected lymphocyte (NAL), percentage of affected lymphocytes (PAL), and diameter (D) of γ-H2AX foci were analyzed (mean ± SD). Differences between M1 and M2 were analyzed using paired t-tests (α = 0.05). Day-to-day variability was evaluated by calculating the coefficients of variation (CV%), bias, and limits of agreement (LoA). Results. There were no statistically significant differences between M1 (N: 7.6 ± 4.4, NAL: 1.2 ± 0.2, PAL: 5.9 ± 2.6%, and D: 0.63 ± 0.07) and M2 (N: 8.4 ± 4.6, NAL: 1.3 ± 0.1, PAL: 6.9 ± 4.2%, and D: 0.66 ± 0.06). CV was calculated to be 98.5% (N), 88.9% (PAL), 11.3% (NAL), and 8.0% (D). Bias (LoA) was 0.75 (−15.2/13.7), −0.02 (−0.36/0.33), −1.0 (−11.9/9.9), and −0.04 (−0.16/0.09), respectively. Conclusions. Background level in healthy subjects is approximately 0.07 to 0.09 γ-H2AX foci/cell. NAL and D are reliable measures.
Highlights
Exercise has been shown to induce an increased formation of reactive oxygen species (ROS) such as superoxide, hydroxyl radicals, or hydrogen peroxide [1, 2]
Day-to-day variability was evaluated by calculating the coefficients of variation (CV%), bias, and limits of agreement (LoA)
There was no difference between measurements of day 1 (M1) and M2 in exercise duration (M1: 1.3 ± 3.5 min/d; M2: 0 ± 0 min/d; P = 0.35) or perceived exertion (M1: 1.4 ± 3.9 ratings of perceived exertion (RPE); M2: 0 ± 0 RPE; P = 0.33) during the 24 hours before the measurements
Summary
Exercise has been shown to induce an increased formation of reactive oxygen species (ROS) such as superoxide, hydroxyl radicals, or hydrogen peroxide [1, 2]. Exercise-induced DNA damage has been observed following acute bouts of exhaustive, prolonged, or eccentric exercises in humans [10,11,12]. Acute bouts of unaccustomed exercises may induce DNA damage, chronic exercises as well as adequate antioxidant intake may alleviate oxidative damage [14,15,16]. Miyazaki et al have shown that twelve weeks of high-intensity endurance training in previously untrained men alleviates oxidative stress induced by an acute session of exercise [15]. Analysis of γ-H2AX foci is a promising approach to evaluate exercise-induced DNA damage. Baseline levels and day-to-day variability of γ-H2AX foci have not been investigated in healthy subjects at rest. Background level in healthy subjects is approximately 0.07 to 0.09 γ-H2AX foci/cell.
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