Abstract

Chronic kidney disease is currently a major public health problem around the world. Although hemodialysis increases survival of patients with end-stage renal disease, kidney transplantation remains the only potentially curative treatment. However, transplantation as a therapeutic option is limited by availability of suitable donor organs. This situation highlights the urgent need to find new and potentially inexhaustible sources of transplantable organs. Perfusion decellularizarion of whole organs is a novel approach to organ engineering and regeneration. In the present research, we used a continuous perfusion decellularization protocol to eliminate cellular componet of kidney and evaluated residual scaffold components after decellularizarion process by proteomics analysis. Our proteomic data show that this protocol results in incomplete removal of cellular proteins. However, unlike other authors, we assume that proteins retained within decellularized kidney scaffold could be the basis for specific homing and celular differentation in the recellularization process.

Highlights

  • Chronic kidney disease (CKD) is currently a major public health worldwide problem [1, 2]

  • Kidney decellularization has been successfully achieved using a variety of approaches and protocols

  • The continuous perfusion decellularization protocol was based on the combination of two detergents (SDS and Triton X-100)

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Summary

Introduction

Chronic kidney disease (CKD) is currently a major public health worldwide problem [1, 2]. CKD affects a significant percentage of the population; mainly due to that their leading causes are highly prevalent disorders such as aging, hypertension, diabetes and vascular disease [3]. The demand for suitable organs for transplantation has reached a level that far exceeds supply. This disparity has greatly to a wide waiting list. These data highlight the urgency to find new and potentially inexhaustible sources of transplantable organs

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