Abstract
To reduce the ambiguity of contradictory observations in different studies regarding the expression level of Macrophage Inhibitory Cytokine-1 (MIC-1) in serum in prostate cancer (PC), benign prostatic hyperplasia (BPH) and healthy controls (HC), we designed this double-blind study. The study comprises 240 sera from PC, BPH and HC subjects. The expression level of MIC-1 in PC, BPH and HC were appraised using Western blot (WB) and ELISA based approach. WB and ELISA appraisal reveals that the expression level of MIC-1 is significantly higher in PC than in HC or BPH subjects. Regression analysis revealed a significant correlation between MIC-1 vs. PSA (r = 0.09; p < 0.001) and MIC-1 vs. GS (r = 0.7; p < 0.001). ROC analysis using discriminant predicted probability revealed that the MIC-1 was better than PSA. Moreover, the combination of MIC-1 and PSA was allowing 99.1% AUC for the differentiation of BPH + PC from HC, 97.9% AUC for differentiation of BPH from HC, 98.6% AUC for differentiation of PC from HC, and 96.7% AUC for the differentiation of PC from BPH. The augmented expression of MIC-1 in PC compared to BPH and HC subjects is in concurrent of the over-expression of MIC-1 in PC reports and confiscates the contradictory findings of other studies.
Highlights
It is usually thought that clinically significant biomarkers may not correspond to the classical serum proteins but are rather instigated from secretion or shedding or leakage of proteins from specific tissue sites[10] and provide the imperative biomarkers to reveal proteins expression changes present at different stages
High-abundance proteins were eliminated as the first step and Western blot (WB) following Enzyme Linked Immunosorbent Assay (ELISA) analysis was performed as the second step
The observation of WB analysis revealed that the expression level of Macrophage Inhibitory Cytokine-1 (MIC-1) was significantly higher in prostate cancer (PC) compared to healthy controls (HC) and benign prostatic hyperplasia (BPH) (Fig. 1)
Summary
It is usually thought that clinically significant biomarkers may not correspond to the classical serum proteins but are rather instigated from secretion or shedding or leakage of proteins from specific tissue sites[10] and provide the imperative biomarkers to reveal proteins expression changes present at different stages. New methods and protocols to decipher low-molecular-weight proteins (LMWP) for PC have been employed and executed to enrich and detect physiologically important LMWP11,12
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
