Abstract
Abstract 1. 1. The release of platelet constituents by thrombin was compared with platelet reactions with connective tissue extract, denatured γ-globulin, Trimeresurus okinavensis venom, and surface-active (lysosome-activating) agents. 2. 2. Thrombin released adenine, nucleotides, but little free amino acids; also small amounts of lipoprotein and protein, with acid phosphatase, β-glucuronidase and procoagulant activities. 3. 3. The venom resembled thrombin in its action. Connective tissue and heat-aggregated γ-globulin released only nucleotides. Surface-active agents, at appropriate concentrations, caused lysis of platelets. 4. 4. The proteins released by thrombin included prealbumin, albumin (34%), β-lipoprotein, platelet-specific α- and β-globulins, fibrinogen and γ-globulin. 5. 5. Lipids released by thrombin were associated with β-lipoprotein and with albumin. The former accelerated clotting of plasma by Russell's viper venom and contained phospholipids including phosphatidyl serine. The latter inhibited clotting and contained mainly neutral lipids. 6. 6. The release reaction caused by thrombin does not appear to be a non-specific extrusion process, or equivalent to lysosome activation. Its mechanism is discussed.
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