Release of Polyanions from Polyelectrolyte Complexes by Selective Degradation of the Polycation

Abstract

One of the major problems associated with analyzing polyelectrolyte complexes is the separation of strongly bound oppositely charged polymeric components. As part of a work aimed at better understanding the factors that affect polyelectrolyte complex formation and stability, an investigation of the possibility to release and analyze the polyanion, after hydrolytic or enzymatic degradation of the partner polycation, was made. Mixtures of poly(acrylic acid) or poly(L-lysine citramide) polyanions with poly(L-lysine) or poly(amino serinate) polycations were investigated. For each polycation-polyanion couple, four complex fractions were obtained by adding the polycation to the polyanion according to a titration protocol. The selective degradation of the polycation within the different complex fractions was investigated after the complex was disrupted with a NaCl solution. The molecular weights of the recovered polyanionic macromolecules were assessed by both static light scattering and size exclusion chromatography. The data supported previous findings that complexation was selective according to the molecular weight of the polyanion for a given polycation. The lower the degree of neutralization of the polyanion negative charges by the polycation positive charges, the greater the molecular weight of the complexed polyanionic macromolecules.