Abstract
After in vivo ligation for 24 h of the cat hypogastric nerve, large amounts of noradrenaline (NA) and dopamine β-hydroxylase (DBH) accumulated in the nerve segment immediately proximal to the ligature (P 1). In vitro incubation of 24-h-ligated nerves (segments P 1 and P 2) in oxygenated Krebs solution at 37% C in the presence of the ionophore X537A or high K + concentrations caused a marked release of endogenously accumulated NA into the incubation medium. High-K +-evoked release was entirely dependent on extracellular Ca 2+. Electrical nerve stimulation caused an 80% tissue NA loss, but the transmitter could not be found in the medium as intact NA. These results suggest that the in vivo ligated cat hypogastric nerve may serve as a useful model of adrenergic nerve terminals free of effector cells.
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