Abstract
This article reports a comparison of the ease of disruption of gram-positive (Lactobacillus acidophilus) and gram-negative (Pectobacterium carotovorum) bacteria using high-pressure homogenization (HPH). The location factor for both enzymes calculated from enzyme release kinetics together with localization studies identified them as cytoplasmic enzymes. The results showed that release of β-galactosidase by HPH of L. acidophilus was more difficult than the release of L-asparaginase from P. carotovorum. It took nine passes at 55.14 MPa for maximum release of β-galactosidase (0.949 IU/mL) as compared to six passes at 41.35 MPa for L-asparaginase (4.653 IU/mL); 1.7 IU of β-galactosidase was released as against 11.5 IU of L-asparaginase per MJ of energy during high-pressure homogenization.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
