Release of internal molecular torque results in twists of Glaucocystis cellulose nanofibers

Abstract

The cellulose of the green alga Glaucocystis consists of almost pure Iα crystalline phase where the corresponding lattice b* axis parameter lies perpendicular to the cell wall surface in the multilamellar cell wall architecture, indicating that in this wall, cellulose is devoid of longitudinal twist. In contrast, when isolated from Glaucosytis cell walls, the cellulose microfibrils present a twisting behavior, which was investigated using electron microscopy techniques. Sequential electron microdiffraction analyses obtained under frozen hydrated conditions revealed that the cellulose microfibrils continuously right-hand twisted in the vitreous ice layer. This observation implies that the twists of these nanofibers are intrinsic to the cellulose molecule and not a result of the cell wall biogenesis process. Furthermore, scaling with the fourth power of width based on the classic mechanics of solid, the twist angle was in agreement with the reported values in higher plant celluloses, implying that the twist arises from the balance between tendency of individual chains to twist and the structure imposed by the crystal packing. The observed twist in isolated fibrils of Glaucocystis indicates that one cannot assume the presence of cellulose twisting in vivo based on observations of isolated cellulose nanoparticles, as microfibril can exist untwisted in the original cell wall but become twisted when released from the wall.