Release of Halophilic Extremozymes by Mechanical Cell Disruption

Mateja Primožič,Maja Čolnik,Željko Knez,Maja Leitgeb

doi:10.17344/acsi.2018.4799

Abstract

Trimatostroma salinum, Wallemia ichthyophaga, Hortaea werneckii and Phaeotheca triangularis are halophilic fungi, which can thrive in a wide range of salinity. They present a source of valuable bioactive compounds, enzymes and proteins interesting for food and pharmaceutical industry. To separate enzymes from halophilic fungi cells, the mechanical method was used. Obtained results and new findings are important from the biotechnological point of view, since the separation of in the form of cocktail from halophilic fungi is interesting for industrial applications, especially for cascade reactions. Enzymes from extremophiles namely possess improved properties and can be used at harsh conditions where non-extremophilic enzymes may deactivate.

Highlights

The great importance of the biotechnology industry is shown in the use of microbial intracellular proteins that possess catalytic or biological activity
The influence of mechanical forces on the viability of H. werneckii, P. triangularis, T. salinum and W. ichthyophaga cells in the cell suspension was a subject of interest (Figure 1)
The greatest resistance to mechanical forces was observed for the H. werneckii cells (D-value = 115.0 min), while the least resistant were the T. salinum cells (D-value = 17.0 min)

Summary

Introduction

The great importance of the biotechnology industry is shown in the use of microbial intracellular proteins that possess catalytic or biological activity. The main drawback is the non-selective release of the products, which complicates the purification process, its price and operating costs Ultrasonication is another common mechanical cell disruption method in which, because of high frequency ultrasound, high shear forces are created which cause cell disruption. Homogenizing the black yeast cell suspensions allows the achievement of more rapid and effective results It is an affordable, simple and reliable method, which enables the extraction of intracellular substances without needing to use solvents or other chemicals to break down the cell walls. Since the studied halophilic fungi are adapted to the high salinity and their cells selectively accumulate compatible solutes such as glycerol, the cell wall of those fungi is more rigid and release of intracellular enzymes can be more difficult These fungi can occur in stressful environments that are hostile to most eukaryotes and are interesting and important for evolutionary processes.

Experimental

Results and Discussion

Conclusion