Abstract
In the present study, the production of a novel feruloyl esterase (FAE) from a typical human intestinal bacterium Lactobacillus acidophilus using various carbon sources was investigated. The results showed that FAE activity was strongly induced by hemicellulosic substances, with the highest activity detected when de-starched wheat bran (DSWB) was used as a carbon source. Moreover, the production was stimulated by the monosaccharides xylose and arabinose, suggesting its particular secretion mechanism. With increasing levels of free ferulic acid (FA) added, the production of FAE increased, reached a peak and declined. Further, on addition of either xylanase or α-L-arabinofuranosidase, the amount of FA released from DSWB by the purified FAE from L. acidophilus increased from 0 to 12.4nmol and 3.64nmol, respectively. When the three enzymes existed together, 15.7nmol of FA was detected. These results indicated that xylanase is predominant and arabinofuranosidase subordinate in their synergistic effect on FA release by FAE.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.