Release of fatty acid-binding protein and long chain fatty acids from isolated rat heart after ischemia and subsequent calcium paradox.

Abstract

To obtain insight into the relation between the release of heart-type fatty acid-binding protein (H-FABPc) and of long-chain fatty acids (FA) from injured cardiac tissue, rat hearts were Langendorff perfused according to the following scheme: 30 min normoxia, 60 min ischemia, 30 min reperfusion, 10 min Ca2+ free perfusion and finally 10 min Ca2+ repletion. During this protocol right ventricular (Qrv) and interstitial effluent samples (Qi) were collected at regular intervals. During reperfusion a total of 0.8 +/- 0.1 nmol H-FABPc but no FA were detected in the effluents. However, during Ca2+ readmission, 45 +/- 4 nmol H-FABPc (80-90% of total tissue content) was released with an initial (first 3 min) simultaneous release of FA (FA/H-FABPc ratio 0.90 +/- 0.07 mol/mol). Thereafter, FA release continued at 10-15 nmol per min mainly in Qrv while the rate of H-FABPc release decreased. During Ca2+ repletion, tissue FA content raised rapidly from 168 +/- 20 to 1918 +/- 107 nmol/g dry weight. These findings suggest that after severe cardiac damage initially FA is released bound to H-FABPc, whereas further FA release occurs in a non-protein bound manner.