Abstract
A rapid transcript increase due to the release of extraction-resistant mRNAs from yeast cells in response to stress is described.
Highlights
As carbon sources are exhausted, Saccharomyces cerevisiae cells exhibit reduced metabolic activity and cultures enter the stationary phase
Stationary phase mRNAs exhibit four patterns of response to oxidative stress To determine whether cells in stationary phase cultures could respond to stress by changes in transcript abundance, cells were harvested at 30 minute time intervals after the addition of 50 μM menadione
A carbon source was not added to ensure that metabolic activity would be low and constant and that any changes in transcript abundance would be due to stress and not to re-feeding
Summary
As carbon sources are exhausted, Saccharomyces cerevisiae cells exhibit reduced metabolic activity and cultures enter the stationary phase. In many cases the signal for quiescence has been identified for both eukaryotes and prokaryotes. In microbes, quiescence is induced in response to various environmental signals. For Saccharomyces cerevisiae, the primary signal appears to be carbon starvation [1,2], other nutrient limitations have been shown to induce a somewhat similar cellular arrest [3]. For all organisms, including prokaryotes, the ability to enter, survive in, and exit this state quickly and efficiently provides a selective advantage over evolutionary time [8] and is highly regulated [1]
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