Release of 6-keto-prostaglandin F 1α and thromboxane B 2 from mouse peritoneal macrophages during their adhesion and spreading on a glass surface

Abstract

The release of 6-keto-prostaglandin F 1α (6KF 1α)_and of thromboxane B 2 (TXB 2) from cells were investigated using mouse peritoneal exudate cells (PECs) and non-cultured peritoneal macrophages. They were prepared by adhesion to glass dishes and incubated for 1 hr at 37°C in 5% Co 2 in air. Both the percentage of spreading macrophages and the release of 6KF 1α and TXb 2 increased in proportion to the incubation time. 6KF 1α and TXB 2 were released from the macrophages, not from the non-adherent cells. When PECs were incubated in silicon-coated glass dishes, the spreading of macrophages was hardly detected and lower amounts of 6KF 1α and TXB 2 were released from these cells compared with cells incubated in non-treated glass dishes. These findings suggest that adhesion with the correlated spreading of macrophages on glass dishes serve as a considerable physical factor for the release of 6KF 1α and TXB 2.