Abstract
Human platelets incubated in platelet-rich plasma (PRP) accumulated [ 14C]5-hydroxytryptamine (5-HT) with a Kt of 2.3/uM and [ 3H]adenine with a K t of 1.1μM. Preincubation with 1.8μM adenine decreased 5-HT uptake by 20%. The incorporated adenine was mainly converted to ATP and ADP. Tyramine, tryptamine, imipramine, reserpine, 1-aminoadamantane (D 1) and its 3.5-dimethyl derivative (D 145) released more than 65% of previously accumulated [ 14C]5-HT. In addition D 145, reserpine,and imipramine also reduced endogenous ATP and ADP within platelets by more than 40% part of which was recovered extracellularly. In[ 14C]adenine-labeled platelets the same 3 drugs caused a marked decrease in adenylate energy charge and [ 14C]ATP which was metabolized mainly to hypoxanthine + inosine. The results indicate that release of 5-HT plus ATP/ADP from platelet storage organelles requires energy which is supplied by the metabolically active adenine nucleotide pool whereas 5-HT alone is liberated rather by passive transport.
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