Abstract
The levels of neurotensin (NT) and its metabolite, the N-terminal octapeptide (NT 1–8), identified by HPLC and measured by RIA, were increased in the hepaticportal circulation of the anesthetized rat during perfusion of the small intestine with a lipid solution, while levels of both peptides remained unchanged in the general circulation. There was no significant arteriovenous difference for NT or NT 1–8 during saline perfusion of the small intestine. Plasma collected from the superior mesenteric vein during the infusion of [ 3H]NT into the superior mesenteric artery showed major peaks of radioactivity with the retention times of NT 1–8 and NT 1–11 on HPLC. Only 12% of the radioactivity recovered from plasma was intact NT. These studies demonstrate that chromatographically identified NT and its metabolite, NT 1–8, are elevated in the portal circulation but not systemic circulation during lipid perfusion and that the small intestine may be both the site of release and metabolism of NT.
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