Relaxin-like gonad-stimulating substance in an echinoderm, the starfish: A novel relaxin system in reproduction of invertebrates

Abstract

Gonad-stimulating substance (GSS) in starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Recently, GSS was purified from the radial nerves of the starfish Asterina pectinifera and its chemical structure determined. This review summarizes the chemical structure of relaxin-like peptide, GSS, from a starfish as the first identified gonadotropin in invertebrates and its hormonal action on reproduction. The starfish GSS is a relaxin-like heterodimeric peptide composed of two peptides (A- and B-chains) with disulfide cross-linkages. Chemically synthesized GSS induced oocyte maturation and ovulation in vitro and an unique spawning behavior followed by release of gametes in vivo. GSS is a first trigger for oocyte maturation in starfish, but its effect is indirect because GSS acts on the ovary to produce a second mediator, 1-methyladenine (1-MeAde), as a maturation-inducing hormone of starfish. The action of GSS on ovarian follicle cells to produce 1-MeAde is mediated through the activation of its receptor, G-protein, and adenylyl cyclase. In contrast to follicle cells in a fully grown state, GSS fails to induce 1-MeAde production in growing follicle cells because of a lack of Gs-proteins. Thus, relaxin-like GSS is a major factor in the neuroendocrine cascade controlling reproduction in starfish.