Abstract
Relaxin immunoactivity levels were measured in the ovaries of rats during various reproductive states with a homologous rat relaxin RIA. Relaxin levels in the ovaries of immature noncycling rats were extremely low, approximately 2 pg/mg equivalent of fresh tissue (FT). Ovarian relaxin concentrations were low throughout the estrous cycle of adult rats but varied with the stage of the cycle. Maximal levels of approximately 20 pg/mgeq FT were obtained at estrus. Relaxin immunoactivity concentrations increased in the ovaries of both ordinary and deciduomata-bearing pseudopregnant rats. In both cases, relaxin levels increased from approximately 160 pg/mgeq FT on day 6 of pseudopregnancy (day 6) to levels ranging from 350–400 pg/mgeq FT on days 10 and 12. After day 12, however, the relaxin concentrations in the ovaries of the two groups of pseudopregnant rats differed. Relaxin levels in the ovaries of ordinary pseudopregnant rats declined by day 15, whereas relaxin levels in the deciduomata-bearing pseudopregna...
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