Relaxed molecular clock provides evidence for long-distance dispersal of Nothofagus (southern beech).

Michael Knapp,David Havell,Karen Stöckler,Federico Sebastiani,Frédéric Delsuc,Peter J Lockhart,Craig Moritz

doi:10.1371/journal.pbio.0030014

Abstract

Nothofagus (southern beech), with an 80-million-year-old fossil record, has become iconic as a plant genus whose ancient Gondwanan relationships reach back into the Cretaceous era. Closely associated with Wegener's theory of “Kontinentaldrift”, Nothofagus has been regarded as the “key genus in plant biogeography”. This paradigm has the New Zealand species as passengers on a Moa's Ark that rafted away from other landmasses following the breakup of Gondwana. An alternative explanation for the current transoceanic distribution of species seems almost inconceivable given that Nothofagus seeds are generally thought to be poorly suited for dispersal across large distances or oceans. Here we test the Moa's Ark hypothesis using relaxed molecular clock methods in the analysis of a 7.2-kb fragment of the chloroplast genome. Our analyses provide the first unequivocal molecular clock evidence that, whilst some Nothofagus transoceanic distributions are consistent with vicariance, trans-Tasman Sea distributions can only be explained by long-distance dispersal. Thus, our analyses support the interpretation of an absence of Lophozonia and Fuscospora pollen types in the New Zealand Cretaceous fossil record as evidence for Tertiary dispersals of Nothofagus to New Zealand. Our findings contradict those from recent cladistic analyses of biogeographic data that have concluded transoceanic Nothofagus distributions can only be explained by vicariance events and subsequent extinction. They indicate that the biogeographic history of Nothofagus is more complex than envisaged under opposing polarised views expressed in the ongoing controversy over the relevance of dispersal and vicariance for explaining plant biodiversity. They provide motivation and justification for developing more complex hypotheses that seek to explain the origins of Southern Hemisphere biota.

Highlights

An important principle of evolutionary inference is that explanations for the past require an understanding of mechanisms and processes applicable in the present [1]
Of all substitution models evaluated, K81ufþG was identified as the best fitting one for the data based on hierarchical likelihood ratio tests and the Akaike Information Criterion. This substitution model and the F84þ C8 model were used for further analyses. The latter was included because the Bayesian relaxed molecular clock (BRMC) approach as implemented in the program MULTIDIVTIME only allows the use of the JC and the F84 models
All nodes of the optimal maximum likelihood (ML) tree recovered in the sensitivity analysis received nonparametric bootstrap support greater than 97%, with the only exception being the grouping of N. cunninghamii with N. moorei, which received 72% support

Summary

Introduction

An important principle of evolutionary inference is that explanations for the past require an understanding of mechanisms and processes applicable in the present [1]. The inference that the seeds of extant Nothofagus species are not suited for dispersal across large distances has played a major role in motivating the hypothesis that transoceanic distributions of Nothofagus (Figure 1) can only be explained by vicariance [11,12,13,14,15]. This hypothesis posits that following the Cretaceous breakup of Gondwana, Nothofagus rafted and evolved in situ upon different Southern Hemisphere lands. Whilst earlier molecular data have been insufficient for rigorous molecular clock analyses, their interpretation has favoured hypotheses of transoceanic dispersal [16,17,18]

Methods

Results

Conclusion