Relaxation optimized double acquisition (RODA) as an alternative for virtual decoupling of NMR spectra

Abstract

We introduce an alternative way for spin-state selection, RODA, which yields higher sensitivity for spin systems exhibiting a TROSY effect. With RODA, the TROSY component of a doublet is recorded twice using a double acquisition scheme. RODA works by simple addition of consecutive NMR signals, and does not require any special processing. Thus, this pulse sequence element can seamlessly be integrated into existing experiments. We demonstrate the broad applicability of RODA with several systems exhibiting a TROSY effect on 15N–1H, 19F–13C or 1H–13C moieties. Further, we show that virtual decoupling with increased sensitivity is possible in a single double acquisition experiment in situations as encountered with dissolution DNP.