Relative sensitivity of brown and rainbow trout to pulsed exposures of an acutely lethal mixture of metals typical of the Clark Fork River, Montana

Abstract

Brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) fry and juveniles were episodically or continuously exposed to a metals mixture (Zn, Cu, Pb, Cd): the concentrations and ratios of the metals, and variations in water quality (pH, hardness), were selected to represent conditions measured during episodic storm events in the Clark Fork River, Montana. Brown trout fry were more sensitive (lower LC50) than rainbow trout fry to the metals in 8-h exposures with constant hardness and pH, but less sensitive to elevated metal concentrations in conjunction with depressed hardness and pH. Fry were more sensitive than juveniles when exposure was continuous, but neither life stage was clearly more sensitive when exposure was pulsed. Whole-body concentrations of K+ and Ca2+ but not Na+ were significantly depressed in fry exposed to metals. Results support the hypotheses that changes in water quality during thunderstorms are lethal to fry and juvenile life stages of brown and rainbow trouts and that the relative sensitivity of the species to the metals mixture may explain their distributions in the Clark Fork River. Low-frequency extreme conditions may effectively act as a bottleneck on the viability of populations whose relative sensitivities to such extremes may control distributions of species in a system.