Abstract
A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC-MS/MS) method was developed for the quantitation of the major metabolite of aconitine, 16-O-demethylaconitine, lappaconitine as the internal standard in rat urine. Urine samples were precipitated with acetonitrile/methanol (3:1, v/v). Chromatographic separation was achieved on a Kromasil C18 analytical column. Detection was performed by a selective reaction monitoring (SRM) mode via an electrospray ionization (ESI) source operating in the positive ionization mode. The analytical method was validated in terms of specificity, precision, accuracy, extraction recovery. The intra- and inter-day precisions were less than 11.7%, and the accuracy was less than 14.0% for the analyte. The validated method has been applied to a pharmacokinetic study of 16-O-demethylaconitine in rats, following oral administration of aconitine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
