Relative importance of climatic and edaphic factors as drivers of plant δ15N along a longitudinal transect

Abstract

ABSTRACT Many studies have shown that climatic and edaphic factors influence the variations in nitrogen isotopes (15N) in terrestrial ecosystems. However, the relative importance of co-varying climatic and edaphic controls on plant 15N remains somewhat unclear. To address this issue, regional-scale plant (L. chinensis) and soil samples were collected along a longitudinal transect across the temperate grasslands in northern China. The influences of climatic and edaphic factors on the plant 15N were disentangled using the variable importance in projection (VIP) approach. We found that the climatic and edaphic variables explained 52.4% and 31.2%, respectively, of the variation in plant 15N. The mean annual precipitation (MAP), soil N, and mean annual temperature (MAT) were the most important variables, but the soil water content (SWC), aridity index (AI), and soil clay content were also important variables. The soil pH and soil C: N ratios were identified as unimportant variables. Furthermore, a high-performance model for simulating plant 15N values was constructed based on the important variables (VIP > 0.8). Further investigations should focus on the effects of the interaction between the climatic and edaphic variables on the N cycles in the temperate grasslands to provide more reliable predictions of plant 15N.