Relative expression of oxytocin receptor gene in buffalo endometrium in late luteal phase and pregnancy stages

Ankita Dilipkumar Verma,Manjit Panigrahi,Bharat Bhushan,Naseer Ahmad Baba,Sourabh Sulabh,Abdul Sadam,Subhashree Parida,Arvind A Sonwane,Krisnaswami Narayanan

doi:10.1080/09712119.2016.1277531

Ankita Dilipkumar Verma, Manjit Panigrahi + Show 7 more

Open Access

https://doi.org/10.1080/09712119.2016.1277531

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Abstract

ABSTRACTMolecular level information related to buffalo (Bubalus bubalis) reproduction and related genes is not present at appropriate level. If such exploration is made in the form of comparison between expression of genes is made between non-pregnant and pregnant phase, it may be helpful to aid manipulate the reproduction. Hence, the present study was carried out to reveal mRNA quantitative real time expression of oxytocin receptor (OTR) mRNA. IFN-τ is considered as the substance of maternal recognition of pregnancy and shut down the probable mechanisms which lead to luteolysis. Such mechanism includes shutting down of OTR. Therefore, relative expression of OTR was studied in endometrial tissue of three groups. The groups were non-pregnant late luteal phase, pregnancy stage I (pregnancy of <42 days) and pregnancy stage II (>42 days of pregnancy). With designed primer and GAPDH as house-keeping gene, relative mRNA expression was measured in Real-time PCR. After statistical analysis of results, the gene found to be expressed in all three stages with non-significant difference.

Highlights

In Bovines, 60–70% of pregnancy losses take place in first three weeks of the pregnancy, the period of pregnancy recognition (Sreenan & Diskin 1983)
Samples from pregnancy stage I were in range of 28–38 days and pregnancy stage II samples were from 48–56 days of pregnancy which was decided on the basis of foetal crown rump/crown vertebral rump length measurement
Relative expression of oxytocin receptor (OTR) mRNA in endometrium during oestrous cycle and pregnancy The amplification plot of targeted region and their dissociation curve is given in Figures 1 and 2

Summary

Introduction

In Bovines, 60–70% of pregnancy losses take place in first three weeks of the pregnancy, the period of pregnancy recognition (Sreenan & Diskin 1983). A signal is sent by conceptus itself in form of interferon-τ (IFN-τ) which ensures maintenance of CL and continued progesterone (P4) production that is must for sustaining pregnancy (Sakumoto et al 2014) In both sheep and cattle, IFN-τ prevents up-regulation of OTR expression in the endometrial luminal epithelium and superficial glandular epithelium (Dorniak et al 2012) as well as development of the pulsatile pattern of PGF2α release needed to attain luteolysis (Wathes & Lamming 1994). The importance of relative expression of OTR is noticeable during oestrous cycle and pregnancy By keeping all these facts, the present study was directed to study relative expression of OTR in endometrial tissue of nonpregnant cyclic (late luteal phase) and pregnant animals. The intension was to reveal the relative expression of OTR between three chosen physiological conditions of reproduction in buffalo

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