Abstract
The aim of this study was to evaluate the relative expression of two candidate genes, CYP (cyclophilin) and Arabinogalactan, possibly involved with seed quality, in contrasting soybean seed coats. The following genotypes were used: BMX Potencia RR and CD 202, both with yellow coats, and TP and IAC, with black coats. The relative expression of the genes was evaluated by qPCR in seven development phases of the coats of the four genotypes at 25; 30; 35; 40; 45; 50 and 55 days after anthesis. The design was completely randomized, with three replicates. The data were submitted to analysis of variance and the means were compared via the Scott Knott test, at 5% probability. The CYP gene showed greater expression in the intermediate stages of development of the coats of the genotypes CD 202 and IAC, yellow coat and black coat, respectively. The A rabinogalactan gene presented greater expression in the final stages of coats development of BMX Potencia RR.
Highlights
In seeds, such as those of soy, there is genetic variability for coat characteristics (DELLAGOSTIN et al, 2011)
Studies have pointed to better performance in seeds of a genotype with a darker coat (MERTZ et al, 2009; SANTOS et al, 2007), it is believed that a number of other factors are involved in seed quality as a function of this outer tissue, since Giurizatto et al (2003) observed that some cultivars with yellow coat presented physiological quality superior to black coat lines
These attributes can be incorporated into cultivars of high productivity, aiming for improvements in the physiological quality of the seed, with the aid of molecular biology tools combined with bioinformatics, in the characterization of genes involved in the control of this attribute and its physiological responses in seeds (HENNING et al, 2009; MOÏSE et al, 2005)
Summary
In seeds, such as those of soy, there is genetic variability for coat characteristics (DELLAGOSTIN et al, 2011). Studies have pointed to better performance in seeds of a genotype with a darker coat (MERTZ et al, 2009; SANTOS et al, 2007), it is believed that a number of other factors are involved in seed quality as a function of this outer tissue, since Giurizatto et al (2003) observed that some cultivars with yellow coat presented physiological quality superior to black coat lines. This reinforces the need for studies at the level of gene expression in order to investigate the genes possibly involved with favorable coat characteristics, even among genotypes with similar color in the aforementioned tissue. These attributes can be incorporated into cultivars of high productivity, aiming for improvements in the physiological quality of the seed, with the aid of molecular biology tools combined with bioinformatics, in the characterization of genes involved in the control of this attribute and its physiological responses in seeds (HENNING et al, 2009; MOÏSE et al, 2005)
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