Relative expression of aldehyde dehydrogenase 1 family member A1 in different malignancies of human glioma cells

Abstract

Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell (CSC) marker that plays a role in the differentiation and metastasis of human glioma cells. In this study, we aimed to analyze the expression of ALDH1A1 in different grades of malignancy of human glioma cells. Samples were collected from 32 patients at Cipto Mangunkusumo Hospital, resulting in 19 low-grade glioma (LGG) specimens, 11 high-grade glioma (HGG) specimens, and 2 normal brain specimens. Isolation of RNA was performed prior to the measurement of the relative quantification of ALDH1A1 by using the quantitative reverse transcription polymerase chain reaction (RT-qPCR). There was a trend of higher overexpression of ALDH1A1 in HGG compared to LGG in humans. However, this finding did not reach the level of statistical significance. ALDH1A1 may be a potential marker for malignancy classification. Therefore, further research is needed.