Relationships between vessel element anatomy and physiological as well as morphological traits of leaves in Fagus crenata seedlings originating from different provenances

Abstract

We attempted to observe differences in vessel element anatomy, and physiological and morphological traits of leaves in Fagus crenata seedlings originated from seven different provenances grown under the uniform environmental conditions. We also investigated the relationships between the anatomical characteristics of the vessel elements and physiological plus morphological traits of leaves in Fagus crenata seedlings. To carry out the experiments, Fagus crenata samples were prepared from Chichibu Research Forest of Tokyo University. For anatomical studies of the vessel elements, vessel number per mm2, average vessel area, and the percentage of vessel area from the pith to the bark side were measured. We also measured transpiration rate, stomatal conductance, leaf area, leaf thickness, leaf dry mass per unit leaf area and leaf density for foliar studies. The anatomical characteristics of the vessel elements as well as the physiological and morphological traits of leaves were noticeably different among provenances. In addition, we found significant correlations between the foliar characteristics not only with vessel number per mm2, but also with vessel area percentage and sum of the (vessel area)2, theoretical hydraulic conductivity, from the pith to the bark side. Therefore, we concluded that variations in physiological and morphological traits of leaves in response to provenance variation were in correspondence with vessel number per mm2 as a parameter that determines the total vessel area and consequent water hydraulic conductance in Fagus crenata as a diffuse porous hardwood.