RELATIONSHIPS BETWEEN THE STRUCTURE AND BIOLOGICAL ACTIVITIES OF CORTICOTROPIN AND RELATED PEPTIDES: BIOLOGICAL ACTIVITIES OF A SYNTHETIC EICOSAPEPTIDE AMIDE.

Abstract

The synthetic eicosapeptide amide of Hofmann, comprising the N-terminal sequence 1 to 20 of corticotropin A1S, in which the twentieth amino acid valine is in the form of the amide, has been investigated for adrenal and extra-adrenal effects by in vivo and in vitro techniques. The adrenal activities assayed were in vivo steroidogenesis in 24-hr hypophysectomized rats and in vitro steroidogenesis by quartered rat adrenals. The in vitro release of free fatty acids by rat epididymal adipose tissue and the mobilization of free fatty acids and the depression of blood sugar in the intact mouse were the extraadrenal effects measured. All activities were assayed relative to corticotropin A1 on a weight basis. The activities of the eicosapeptide amide in the in vitro assays were steroidogenesis, 181%, adipokinetic effect, 160%, and hypoglycemia, 200%. By in vitro techniques the steroidogenic effect was 78% and the adipokinetic effect was 39%. Thus, by in vivo assays the eicosapeptide amide has the same molar activi...