Relationships between the level of atresia and inhibin contents (alpha subunit and alpha-beta dimer) in morphologically dominant follicles during their growing and regressing phases of development in cattle.

Abstract

The objectives were to determine the relationships between changes in the levels of histological and biochemical (estradiol [E2]:androstenedione [A], E2:A ratio) atresia and changes in inhibin contents of morphologically dominant follicles collected during the growing or the regressing phase of the first wave of follicular development in cycling crossbred beef heifers. Heifers were slaughtered either when the dominant follicle (> or = 9 mm; diameter of the antral cavity as assessed by ultrasonography) of the first wave was still growing (n = 7) or when the first dominant follicle (> or = 9 mm; n = 7) was regressing or was at the end of the plateau phase. Following ovary collection, the dominant follicle was dissected and level of histological atresia was determined on sections of follicular walls. Aliquots of follicular fluid from each of the dominant follicles were collected to measure concentrations of E2, A, and inhibin alpha subunit by RIA and to measure concentrations of dimeric (alpha-beta dimer) inhibin by a two-site immunoradiometric assay. Heifers were slaughtered on Days 5.4 +/- 0.5 (growing phase) and 11.8 +/- 0.5 (regressing phase) of the estrous cycle, and mean diameter of the dominant follicles was similar in both phases (9.9 +/- 0.4 vs. 10.8 +/- 0.4 mm; p > 0.09). All morphologically dominant follicles collected during the growing phase (7/7) were histologically healthy and estrogen-active (E2:A ratio > 1), while those collected during the regressing phase (7/7) were histologically atretic and estrogen-inactive (E2:A ratio < 1; chi 2 = 14.0, p < 0.0001).(ABSTRACT TRUNCATED AT 250 WORDS)