Relationships between the isotopic composition of dissolved organic carbon and its bioavailability in contrasting Ozark streams

Abstract

Bioavailability of dissolved organic carbon (DOC) can significantly influence nutrient cycling in small streams yet the factors influencing the bioavailability of DOC remains poorly understood. The isotopic composition of DOC and factors controlling its utilization were studied in two contrasting headwater streams to elucidate the relationship between DOC source and its bioavailability. Water samples were collected monthly from Moore Creek (MC), located in a watershed dominated by fertilized pasture, and from Huey Hollow (HH), located in a deciduous forest watershed. Dissolved organic carbon concentrations ranged from 222 to 1130 μm C and 35 to 289 μm C in MC and HH, respectively. The isotopic composition of DOC (δ13CDOC) was more seasonally variable in HH and ranged from −33.6 to −28.0Ğ, as compared with MC where it ranged from −27.2 to −24.5 Ğ. The δ13CDOC in Huey Hollow suggests leaf debris was an important source of DOC throughout most of the year while algae was important in winter and early spring. In MC, the δ13CDOC indicated DOC was largely derived from soil organic matter while algal inputs were small relative to the large pool of refractory DOC. Stream water community respiration (SWCR) rates suggest the existence of a larger pool of refractory DOC in MC relative to HH. The ratio of SWCR (μM C h−1) to DOC concentration (mM C) was generally higher in HH (1.2–13.2) as compared with MC (0.2–4.2), suggesting that relative bioavailability of DOC was often greater in HH. Nutrient addition experiments indicate that bioavailable C limited SWCR in spring and fall in MC, while bioavailable C was never limiting in HH. The results suggest that elevated nutrient and DOC concentrations supported higher levels of microbial activity that resulted in a large pool of refractory DOC in MC. The lower inorganic nutrient and DOC concentrations reduced microbial activity in HH and likely limited the production of refractory DOC. Results of this study suggest that both organic matter source and nutrient concentration are important determinants of DOC bioavailability in small streams. %