Relationships between soil macroaggregation and humic carbon in a sandy loam soil following conservation tillage

Abstract

Humic substances are recalcitrant and might act as persistent binding agents to form macroaggregates. The focus of this study is in investigating the contribution of humic carbon (HC) to soil aggregation in response to various tillage and residue managements. Arable soils following 8-year contrasting managements were collected to determine aggregate size distribution and stability and HC fractions including humic acid (HA) and fulvic acid (FA). The contribution of HC to aggregation was divided into three special effects including positive effect (PE), negative effect (NE), and combined effect (CE), and these effects were measured using aggregate fractionation techniques. As well as to promote structural stability, HC bounds predominantly with the silt + clay fraction and secondarily with microaggregates to form larger aggregates. The PE increased with increasing aggregate size, whereas the NE followed the opposite pattern. A positive CE was observed for large and small macroaggregates, whereas the CE for microaggregates and the silt + clay fraction was negative. Compared to continuous tillage, reduced- and no-tillage decreased the PE for large and small macroaggregates by 1.58–30.98% at the 0–20 cm depth, and straw returning also slightly decreased the corresponding PE relative to straw removing. By contrast, a significantly higher NE for small macroaggregates at the 0–10 cm depth while 6.33–81.11% decreases in CE for large and small macroaggregates at the 0–10 cm depth as well as for large macroaggregates at the 10–20 cm depth, were observed under reduced- and no-tillage. The extraction of HC significantly reduced the aggregate stability and reduced- and no-tillage effectively limited its decrease magnitude. Small macroaggregates and microaggregates made larger contributions to soil HC accumulation than did other fractions. An averagely increased contribution from large or small macroaggregates was observed under both reduced-/no-tillage and straw returning at the 0–20 cm depth. A significant and positive relationship was found between the mass proportion of macroaggregates and the HC accumulation in 0–20 cm soil. Large macroaggregates had significantly higher HA/FA ratios than small macroaggregates, and reduced- and no-tillage significantly increased these ratios both in large and in small macroaggregates. The CE for large or small macroaggregates was also significantly negatively correlated with their HA/FA ratios. Overall, the HC accumulation in soil is likely to play a key role in macroaggregation, but conservation tillage might decrease the contribution magnitude of HC to large or small macroaggregation through increasing the corresponding HA/FA ratios.