Relationships between Single Nucleotide Polymorphism Markers and Meat Quality Traits of Duroc Breeding Stocks in Korea.

J S Choi,J H Jung,Y H Jeong,Y I Choi,S K Jin,Y C Jung,K S Shim

doi:10.5713/ajas.16.0158

Abstract

This study was conducted to determine the relationships of five intragenic single nucleotide polymorphism (SNP) markers (protein kinase adenosine monophosphate-activated γ3 subunit [PRKAG3], fatty acid synthase [FASN], calpastatin [CAST], high mobility group AT-hook 1 [HMGA1], and melanocortin-4 receptor [MC4R]) and meat quality traits of Duroc breeding stocks in Korea. A total of 200 purebred Duroc gilts from 8 sires and 40 dams at 4 pig breeding farms from 2010 to 2011 reaching market weight (110 kg) were slaughtered and their carcasses were chilled overnight. Longissimus dorsi muscles were removed from the carcass after 24 h of slaughter and used to determine pork properties including carcass weight, backfat thickness, moisture, intramuscular fat, pH24h, shear force, redness, texture, and fatty acid composition. The PRKAG3, FASN, CAST, and MC4R gene SNPs were significantly associated with the meat quality traits (p<0.003). The meats of PRKAG3 (A 0.024/G 0.976) AA genotype had higher pH, redness and texture than those from PRKAG3 GG genotype. Meats of FASN (C 0.301/A 0.699) AA genotype had higher backfat thickness, texture, stearic acid, oleic acid and polyunsaturated fatty acid than FASN CC genotype. While the carcasses of CAST (A 0.373/G 0.627) AA genotype had thicker backfat, and lower shear force, palmitoleic acid and oleic acid content, they had higher stearic acid content than those from the CAST GG genotype. The MC4R (G 0.208/A 0.792) AA genotype were involved in increasing backfat thickness, carcass weight, moisture and saturated fatty acid content, and decreasing unsaturated fatty acid content in Duroc meat. These results indicated that the five SNP markers tested can be a help to select Duroc breed to improve carcass and meat quality properties in crossbred pigs.

Highlights

Duroc boar is used as a terminal sire at commercial pig production
On processed yield and meat quality as the gene regulates After pigs per pen reached the market weight of 110 kg on glycogen content in muscle (Milan et al, 2000). They were slaughtered following the guidelines of al. (2001) found the PRKAG3 allele in several pig breeds, Livestock Products Sanitary Control Act (Ministry of Food including Landrace, Large White, Berkshire, Duroc, and and Drug Safety in Korea, 2010), and chilled overnight in a 4°C cold room
Fatty acids: Total lipids of samples were extracted by using chloroform-methanol (2:1, v/v) according to the procedure of Folch et al (1957)

Summary

INTRODUCTION

Duroc boar is used as a terminal sire at commercial pig production. This breed has an excellent growth rate and higher intramuscular fat content than other breeds (Suzuki et al, 2003). On processed yield and meat quality as the gene regulates After pigs per pen reached the market weight of 110 kg on glycogen content in muscle (Milan et al, 2000). Ciobanu et average, they were slaughtered following the guidelines of al. A total of five and Ser638Arg) located on the swine chromosome 2 region candidate gene (PRKAG3, FASN, CAST, HMGA1, and were related to the tenderness of longissimus dorsi (LD) MC4R) polymorphisms were previously reported and muscle in pigs. Digested PCR products were analyzed on a 2.5% to 4% agarose gel, and each allele was scored

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION

CONFLICT OF INTEREST