Abstract
1. 1. Hematocyte lysates from lobster (Homarus vulgaris) and crayfish (Astacus leptodactylus) were prepared under pyrogen-free conditions in the presence of N-ethyl-maleimide and then acidified. 2. 2. In both lobster and crayfish lysates, one hemocyanin-related fraction and at least one hemagglutinin fraction were delected. 3. 3. Plasma coagulogen was not found inside the cells, except in the presence of cysteine hydrochloride. When present, it appeared to be linked to a pink cellular agglutinin. 4. 4. Activation of cellular clotting by lipopolysaccharides or β-1,3 glucans showed that most hemocyte proteins are involved in the coagulation process. A factor which dissolved in acidic medium and moved towards the cathode was observed, as well as urea-soluble proteins. The first factor might be a Limulus-like cellular coagulogen. 5. 5. The relationships between the humoral defense factors plasma coagulogen, agglutinins, phenoloxidase, and opsonins are discussed.
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